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KMID : 0380020150300060332
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Korean Journal of Biotechnology and Bioengineering
2015 Volume.30 No. 6 p.332 ~ p.338
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Pretreatment and Enzymatic Saccharification of Wasted MDF for Bioethanol Production
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Kang Yang-Rae
Hwang Jin-Sik
Bae Ki-Han
Cho Hoon-Ho
Lee Eun-Jeong
Cho Young-Son
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Abstract
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The objective of this study was designed to determine the possibility of bioethanol production from wasted medium density fiberboard (wMDF). We were investigated the enzymatic saccharification characteristics using the enzyme (Cellic CTec3) after pretreatment with sodium chlorite. According to the component analysis results, the lignin contents before and after the pretreatment of wMDF (milling using sieve size of 1,000 μm) was significantly reduced from 31.13% to 4.11%. Therefore, delignification ratio of pretreated wMDF was found to be up to about 87-89% depending on the sieve size. And we were tested to compare the saccharification ratio according to the sieve size of wMDF (1,000 μm, 200 μm), but it was no significance depending on the sieve size. When enzyme dosage was 5% based on the substrate concentration, enzymatic saccharification ratio was obtained up to 70% by maintaining at 50°C for 72 hours. We could made the substrate concentration of pretreated wMDF (1,000 μm) up to 12% and then enzymatic saccharification ratio was 76.8%, also contents of glucose and xylose were analyzed to 77,750 and 14,637 mg/L, respectively.
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KEYWORD
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MDF, Pretreatment, Saccharification, Bioethanol
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